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Representation of an amino acid (the thick green and red structure) in the active site of the new tRNA synthetase generated by Wang et al. The mutated amino acids (shown in yellow) allow the incorporation into newly synthesized peptides of the unconventional amino acid O-methyl-L-tyrosine, rather than the tyrosine shown.
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NeoGenesis: How Scientists Are Creating Alternate Life Forms
By Robert Roy Britt
Senior Science Writer
posted: 07:00 am ET
08 May 2001

neogenesis_010508

Cloning, move over.

The powerful and potentially dark force that would give you a "mini-me" may soon be knocked off genetic engineering's center stage by an emerging laboratory effort to create life that is decidedly not as we know it.

In two separate research efforts, scientists have altered the very nature of nature by creating cells that break a cardinal rule of biology, incorporating an entirely new basic building block into their cellular structures. It is a first step on a path of neogenesis -- the creation of alternate life forms.

In several interviews, experts said the work will help scientists study our own terrestrial evolution and also investigate how life might have developed in unknown ways on other worlds.

Here's how it is happening:

All life is made up of cells built and operated by proteins, which in turn are made from 20 building blocks called amino acids. No one knows why only 20 are used, but that is an unbroken rule in all of biology throughout the history of life on Earth, from the smallest bacteria to the prettiest flower to the largest WWF wrestler.

By rewriting the genetic instructions inside bacteria, two separate research groups tricked the one-celled microscopic critters into incorporating a hitherto unused amino acid into the process of building proteins.

The work is reported in the April 20 issue of the journal Science.

AT, not ET

Scientists said that creating a laboratory ET is a ways off, assuming humanity even agrees to go there.

But the novel cells are already on the verge of alternative life, said a scientist who worked on one of the studies, Paul Schimmel of the Scripps Research Institute in California.

"It is a first step to creating organisms that use building blocks other than the ones we are all familiar with," Schimmel told SPACE.com. "In that sense, you could consider it an alternative life form."

Schimmel said the bacteria survive and reproduce, even though their proteins are chugging along with an alien amino acid.

"Maybe AT (artificial terrestrial life) would be a better term," for what the new research has accomplished, said Michael A. Meyer, an astrobiologist at NASA Headquarters and program scientist on the recently launched Mars Odyssey mission.

While the work's initial impact will likely come in the form of novel drugs and revolutionary new materials, its effects could reverberate through the study of our origins and the search for life.

How to study ET without leaving home

Astrobiologists, chemists and other scientists say this ability to tinker with biology will provide new ways to investigate how life began on Earth and how it evolved. It might also help determine whether extraterrestrial life could follow different rules of biology.

Schimmel said the result raises the possibility that unusual amino acids in space could provide the building blocks of unimagined living systems, and that "a more primitive code could be used to get life started."

David W. Deamer, a biochemist at the University of California Santa Cruz, said the results "confirm that the life process is not a perfect machine, but instead a molecular system that will use whatever it can find as long as it works."

This apparent flexibility in how modern bacteria structure themselves "can be explored to determine the limits of life in adapting to novel structures, as must have also occurred on the early Earth at the time that life began," Deamer said.

And if life forms are ever found on Mars or elsewhere, scientists would now be better equipped to determine whether a true alien has been found or whether we've just met back up with ancient ancestors that somehow traveled from one celestial body to another.

"Suppose we get to Mars or Europa and find living microorganisms," Deamer said. "How will we know whether they are true ET or simply delivered there from the Earth? Or vice versa?

"If they have a different genetic code and use different amino acids...we might conclude that life began independently. But if we can force terrestrial bacteria to evolve toward such a different set of [building blocks] and codes, it will suggest that the life process has much more flexibility than we think, so that a true ET could solve the same basic puzzle when it originated, but use different pieces."

Next page: Editing errors; Another dark force?

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